proteome profilertm human angiogenesis antibody array  (R&D Systems)

Journal: Viruses

Article Title: Human Brain Microvascular Endothelial Cells Exposure to SARS-CoV-2 Leads to Inflammatory Activation through NF-κB Non-Canonical Pathway and Mitochondrial Remodeling.

doi: 10.3390/v15030745

Figure Lengend Snippet: Figure 4. Production of angiogenic-related molecules is modulated by SARS-CoV-2 in HBMECs. (A) Conditioned medium from Mock and SARS-CoV-2-exposed HBMEC cultures (both with MOI 0.01 and 0.1) were analyzed via Proteome Profiler Human Angiogenic Antibody Array and detected by chemoluminescence, each protein detected in duplicated spots. (B) Densitometric analysis of membranes in (A) revealed the analytes with the strongest signal and which were affected by the SARS-CoV-2 challenge. Spots labelled 1-15 in (A) correspond to the analytes depicted in (B). (C) RT-qPCR analysis of angiogenesis-related genes in HBMECs revealed that PTX3 and HIF-1α were increased following SARS-CoV-2 exposure. *: p < 0.05; **: p < 0.01, two-way ANOVA with Bonferroni post-test of at least five independent experiments.

Article Snippet: Secretion of angiogenesis-related protein levels was detected using a Proteome ProfilerTM Human Angiogenesis Antibody Array kit (R&D Systems) according to the manufacturer’s instructions.

Techniques: Ab Array, Quantitative RT-PCR